Poliner et al., 2017 constructs
These constructs will be available soon from Addgene
| Vector name | Use | Selection in target organism |
|---|---|---|
| for Nannochloropsis | ||
| pNOC-OX-HA | Tagged overexpression, 1 gene | Hygromycin |
| pNOC-OX-CFP | Tagged overexpression, 1 gene | Hygromycin |
| pNOC-OX-YFP | Tagged overexpression, 1 gene | Hygromycin |
| pNOC-OX-GFP | Tagged overexpression, 1 gene | Hygromycin |
| pNOC-OX-Nlux | Tagged overexpression, 1 gene | Hygromycin |
| pNOC-411 | Basic transformation construct | Zeocin |
| pNOC-401 | Basic transformation construct | Hygromycin |
| pNOC-401-P2A(60)-Hsterm | Overexpression linked to resistance marker P2A line | Hygromycin |
| pNOC-411-P2A(60)-Hsterm | Overexpression linked to resistance marker P2A line | Zeocin |
| pNOC-411-P2A(60) | Overexpression linked to resistance marker P2A line | Hygromycin |
| pNOC-401-P2A(60) | Overexpression linked to resistance marker P2A line | Zeocin |
| pNOC-401-P2A(60)-Flux | Expression by resistance marker P2A firefly luciferase line | Hygromycin |
| pNOC-411-P2A(60)-Flux | Expression by resistance marker P2A firefly luciferase line | Zeocin |
| pNOC-stacked-HygR-Nlux | Tagged overexpression, 2 genes | Hygromycin |
| pNOC-stacked-BleR-GFP | Tagged overexpression, 2 genes | Zeocin |
| pNOC-DOX9 | HA tagged expression of delta-9 FAD | Zeocin |
| pNOC-stacked-DOX9+12 | HA tagged expression of delta-9 FAD, and HA tagged delta-12 FAD | Zeocin |
| pNOC-DOX12-HA | HA tagged delta-12 FAD expression | Hygromycin |
| pNOC-DOX12-CFP | CFP tagged delta-12 FAD | Hygromycin |
| pNOC-DOX5-CFP | CFP tagged delta-5 FAD | Hygromycin |
| for yeast | ||
| pESC-leu-D5+w3 | Expression of N. ocenica delta-5 and w3 in yeast | leucine auxotrophy |
| pESC-his-D12+D6 | Expression of N. ocenica delta-12 and delta-6 in yeast | histidine auxotrophy |
| pYES-E6 | Expression of N. ocenica elongase-6 in yeast | uracil auxotrophy |